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Friday, March 27 • 08:00 - 17:00
[VIRTUAL] Characterization of Novel Promoter Sequences for Use in Geobacillus thermoglucosidasius

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This project’s purpose is to characterize novel promoters for expression in Geobacillus thermoglucosidasius with GBK1 and GBK2 bacteriophages in order to expand the uses of G. thermoglucosidasius in biological research. Determining new sequences for transformation expands the possible proteins, enzymes, and characterization which can be expressed. Once promoters were identified, primers were designed for use with pG1AKmob vector. After successful ligation, products were transformed by conjugation and the GFP expression was measured. The final conjugated Geobacillus thermoglucosidasius cells grew on Ampicillin and Kanamycin plates, with minor expression of sfGFP when visualized under UV light.


Friday March 27, 2020 08:00 - 17:00 EDT