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Identifying microorganisms that naturally metabolize and break-down hydrocarbons, can reduce pollution created from the use of crude oil through bioremediation. For our work, bacteria were collected from soil/oil and oil drip pan deposits from an automobile oil. The bacteria were isolated and its morphology and Gram reaction were determined. Then, colony PCR was performed using 16S ribosomal RNA (rRNA) to identify its genus and species. To confirm the bacteria’s ability to metabolize hydrocarbons, the bacteria's growth was quantified on different growth media/environments.
