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Aromatic compounds found in wastewater are often identified as potential contaminants. Bacteria used in the treatment process break down these compounds, but the metabolic process is unclear. The goal of this project is to determine if bacteria from Harnett County Wastewater Treatment Facility are capable of degrading the aromatic compound lignin. Wastewater samples were plated on media containing lignin. The bacteria were identified using polymerase chain reaction (PCR) to amplify the 16S rDNA gene. Sequencing was used to determine the identity of the bacteria capable of lignin degradation. Future testing will focus on enzymatic characterization of bacterial lignin degradation.
